Combination of Black Cumin Seeds with Curcuma xanthorrhiza Extract as an antioxidant and immunomodulator agent in the Covid-19 Pandemic Era

Akrom Akrom, Titiek Hidayati, Nurcholid Umam, Ginanjar Zuhruf Saputri


The COVID-19 pandemic demands changes in using of medicinal plants. Empirically, the medicinal plants of Curcuma xanthorrhiza (CX) and black cumin (BC) have been used massively in the era of the covid-19 pandemic. CXBC preparations have been developed with the main ingredients of BC oil and CX extract. The purpose of the study was to determine the content of active substances (polyphenols, flavonoids, thymoquinone), antioxidant and immunomodulatory activities of CXBC preparations. We conducted experimental laboratory research. The immunomodulatory activity test was carried out on THB-183 cells by observing the expression of TNF-α and IL-10. The results showed that the CXBC preparation contained 4% thymoquinone, 25.87 mg/ml polyphenols, and 41.86 mg/dl flavonoids. CXBC preparations contain vitamins (A, C, and E) and minerals (potassium, calcium). The antioxidant activity of the CXBC preparation was included in the strong category with IC50 = 54.87 ppm. CXBC preparations increased TNF-a expression and decreased IL-10 expression in THB-138 cells. Based on the study results, it can be concluded that the CXBC preparation contains 4% thymoquinone, 25.87 mg/ml polyphenol, 41.86 mg/dl flavonoid, and a high level of vitamin and minerals. CXBC preparations have potent antioxidant activity, increase TNF-α and decrease IL-10 expression.


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