The Effectiveness of Chitosan Membrane from Blood Clam Shell (Anadara granosa) in Reducing Total Coliform Bacteria in Household Grey Water

Iva Rustanti Eri, Marlik Marlik, Darjati Darjati, Yudied Agung Mirasa

Abstract


Clamshell is a solid trash that has a rough texture, difficult to digest, and polluting the environment. Blood clam shells (Anadara granosa) contain chitin, which is converted into antimicrobial chitosan. The purpose of this study was to see the effectiveness of chitosan membrane from blood clam shells in reducing total coliform bacteria in household grey water. This study was carried out using experimental research method design.  This research focused on household grey water waste. The chitosan concentrations on the membrane varied between 2.5% and 3.5% with filtration times of 20 and 60 minutes. The Anova Quadratic Model test was further used to examine the data. Chitosan was separated from the shell of the blood clam at peak angles of 19.66° and 26.04° according to XRD characterization. It was obtained that chitosan membrane, with a concentration of 3.5% and a filtering duration of 60 minutes, provided the highest percentage of total coliform bacteria reduction of 99.9%. The conclusion of this study is that blood clamshells used as chitosan membranes can reduce total coliform bacteria in household grey water waste. 

References


Agustina, S., Swantara, M and Suartha, N., "Isolation of Chitin, Characterization, and Synthesis of Chitosan from Shrimp Shell," Journal of Chemistry Udayana University, 2(9):271-278, 2015. DOI: https://doi.org/10.24843/JCHEM.2015.v09.i02.p19,2015.

Butarbutar, E., "Antibacterial Activity Test of Chitosan Made from Crab Shell (Portunus pelagicus) Against Staphylococcus aureus and Escherichia coli," North Sumatera University, 2018. Received from : http://repositori.usu.ac.id/handle/123456789/6677.

Darjati and Marlik, "Mussel Shell Powder as Bio-adsorbent of Heavy Metals in Water," Health Nations, 2(1):40-44, 2018. DOI: https://doi.org/10.33846/hn.v2i1.105, 2018.

Darjati, Marlik and Demes,N., "The Mussel Shell Powder as Natural Preservation for Swordfish by Using Smearing Method," Health Nations, 2(2):249-251, 2018. DOI: https://doi.org/10.33846/hn.v2i2.124, 2018.

Estiasih, T., Ahmadi, E., Kurniawati, D and Ginting, "Optimization of Lecithin Extraction Yield from Soybean Oil of Anjasmoro Variety with Water Degumming," Journal of Food Technology and Industry, 24(1):97-104, 2013. DOI: https://doi.org/10.6066/jtip.2013.24.1.97, 2013.

Hidayat, T., "Synthesis of Chitosan - PEG (Polyethylene Glycol) Membrane and Its Application as an Adsorbent for Cr6+ Ions and Ni2+ Ions in Solution," Semarang State University, 2015. Received from : http://lib.unnes.ac.id/id/eprint/20530.

Ilhamudin, M., "The Effect of Mangrove Density Levels on Growth and Survival of Blood Clams (Anadara granosa)," Journal of Fisheries Mataram University, 9(1):75-85, 2019. DOI: https://doi.org/10.29303/jp.v9i1.142, 2019.

Karina, I., Fadli, A and Drastinawati, "Demineralization reaction kinetics on chitin isolation from shrimp shell waste from ebi industry," Journal of Engineering Faculty Riau University, 4(1):1-4, 2017. Received from : https://jom.unri.ac.id/index.php/JOMFTEKNIK/article/view/14534.

Kurniasih, M and Kartika, D, "Antibacterial Activity of Chitosan Against S. aureus Bacteria," Journal of Molecular Jendral Soedirman University, 4(1):1-5, 2018. DOI: http:/dx.doi.org/10.20884/1.jm.2009.4.1.56, 2018.

Lubis, R and Usman, M., "Utilization of Chitosan from Shells Waste (Anadara inflata) as River Water Purifier," 2016. Received from : https://ojs.uma.ac.id/index.php/biolink/article/view/16.

Laluce, C., Tognolli, J.O., Olivieria, K.F.D, Souza, C.S and Morais, M.R, "Optimization of Temperature Sugar Concentration and Inoculum Size to Maximize Ethanol Production without Significant Decrease in Yeast Cell Viability," Journal of Applied Microbiology and Biotechnology, 83:627-637, 2009. DOI: 10.1007/s00253-009-1885-z, 2009.

Magani, A.K., Tallei, T.E and Kolondam, B.J., "Antibacterial Test of Chitosan Nanoparticles on the Growth of Staphylococcus aureus and Escherichia coli," Journal of Bios Logos Sam Ratulangi University, 10(1):7, 2016. DOI: https://doi.org/10.35799/jbl.10.1.2020.27978, 2016.

Masindi, T and Herdyastuti, N., "Characterization of Chitosan from Blood Shells (Anadara granosa)," Journal of Chemistry, 6(3):137-142, 2017. Received from : https://jurnalmahasiswa.unesa.ac.id/index.php/unesa-journal-of-chemistry/article/view/21997.

Pamela, VY., Syarief, R., Iriani, and Suyatma, NE., "Mechanical, Thermal, and Morphological Characteristics of Polyvinyl Alcohol Films With the Addition of ZnO Nanoparticles and Acetic Acid For Multilayer Packaging," Journal of Agricultural Postharvest Research, 13(2):63, 2017. DOI: http://dx.doi.org/10.21082/jpasca.v13n2.2016.63-73, 2017.

Pursetyo, K.T., Tjahjaningsih, W., and Pramono, H., "Comparative Morphology of Blood Clams in Kenjeran and Sedati Waters," Scientific Journal of Fisheries and Marine Affairs Airlangga University, 7(1):31-33, 2015. DOI: http://dx.doi.org/10.20473/jipk.v7i1.11222, 2015.

Rafiqa, N., Eri, I., Demes N., Ferry, K and Narwati, "Ability of chitosan membrane from blood clam (Anadara granosa) shells to removal iron level (Fe) in water," International Journal of Public Health Science, Vol. 10, No. 2, June 2021, pp. 330-335, 2020. DOI: 10.11591/ijphs.v10i2.20682, 2021.

Ramadhanur, S., and Sari, A.M., "'The Effect of Chitosan Concentration and Filtration Time of Chitosan Membranes," Journal of Conversion Muhammadiyah University Jakarta, 4(1):40-52, 2015. DOI: https://doi.org/10.24853/konversi.4.1.%25p, 2015.

Sarwono, R., "Utilization of Chitin / Chitosan as Anti-Microbial Material," International Journal of Applied Chemistry, 12(1):32-38, 2010. DOI: https://doi.org/10.14203/jkti.v12i1.150, 2010.

Solang, M., Blood Shellfish: Don't Know So Unhealthy," Zahir Publishing, 2019, ISBN: 978-602-5541-64-3.

Stephen, A.M., Philips, G.O and Williams, P.A., "Food Polysaccharides and Their Applications: Second Edition," CRC PRESS, 2016, ISBN: 9780824759223.

Stevano, V., Bayu Andhika and Isna Syauqiah, " Utilization Of Chitosan From Waste Shell Shelts As Adsorbent Of Heavy Metal Zinc (Zn)," Journal of Conversion , 5(1):22-26, 2016. DOI: http://dx.doi.org/10.20527/k.v5i1.4775, 2016.

Sulistia, S., and Septisya, A.C., "Analysis of Domestic Office Wastewater Quality," Journal of Environmental Engineering Bogor Agricultural University, 12(1):41-57, 2019. DOI: https://doi.org/10.29122/jrl.v12i1.3658, 2019.

Wahyuni, S., Khaeruni and Hamidah, "Application of chitosan membrane from tiger prawn shells (Penaeus monodon) to extend the shelf life of sweet orange juice (Citrus sinensis)," Journal of Food Science and Technology Halu Oleo University, 2(1):272-284, 2017. DOI: http://dx.doi.org/10.33772/jstp.v2i1.2111, 2017.

Wahyuni, S., Siswanto, S., and Putra, S.M., "Formulation of Chitosan Membrane Composition and Optimization of Stirring in Reducing the Solid Content of Palm Oil Liquid Waste," Widyariset, 3(1):35, 2017. DOI: 10.14203/widyariset.3.1.2017.35-46, 2017.

Yacika, R., Adhitama, R., Barat, D., and Graha, P., "Anadara granosa Shell as Antibacterial Biocoating on Textile," Journal of Center For Young Scientists, 2015. DOI: 10.13140/RG.2.2.29765.81125, 2015.

Yatul, F., " PVDF/TiO2/PEG Hollow Fiber Membrane for Oily Wastewater Treatment at Various Concentration of Oily Wastewater," International Seminar of Science and Technology, 2015. DOI: http://dx.doi.org/10.12962/j23546026.y2015i1.1122, 2015.

Younes, I., and Rinaudo, M., "Chitin and chitosan preparation from marine sources. Structures, properties and applications," National Library of Medicine, 13(3):1133-1174, 2015. DOI: 10.3390/md1303113.

Zhang, Z., Chen, Ji, Huang Y., and Chen, D., "Antibacterial Properties of Cotton Fabrics Treated with Chitosan," Journal of Textile Research, 73:1103-1106, 2003. DOI: https://doi.org/10.1177/004051750307301213, 2003.




DOI: http://doi.org/10.11591/ijphs.v11i3.21291

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