Bacterial and parasitic contamination of raw vegetable in Jember regency, Indonesia : potential risk for food-borne diseases

Diana Chusna Mufida, Elvia Rahmi Marga Putri, Dini Agustina, Enny Suswati, Muhammad Ali Shodikin, Yunita Armiyanti, Bagus Hermansyah, Wiwien Sugih Utami, Angga Mardro Raharjo

Abstract


A raw vegetable is one of the transmission factors in human food-borne infection. Contamination might start from the plantation, distribution, until at serving time in the dining table. A higher level of contamination will increase the risk of food-borne illness. The study aims to determine bacteria and parasite contamination in some raw vegetables that people usually consume in Jember Regency, East Java, Indonesia. In this study, we collected raw vegetable from eight traditional markets. We chose randomly at each market five samples of vegetables that usually directly consumed, like lettuce, tomato, cabbage, basil, long bean, and cucumber. Pathogens we identified limited on bacteria and parasite like helminth and protozoan group. This study showed that 91% contaminated by E. coli, 84% by Staphylococcus aureus, and 79% contaminated by Vibrio cholerae. Besides bacteria, 36% of samples contaminated by the helminth group, and protozoa contaminated 27%. The most vegetables contaminated was lettuce, even in bacterial or parasitic contamination. This study concluded that there is contamination in raw vegetables usually directly consumed in Jember Regency. It will be a potential risk factor for food-borne illness in the future.

References


M. F. Lynch, R. V. Tauxe, and C. W. Hedberg, “The growing burden of foodborne outbreaks due to contaminated fresh produce: Risks and opportunities,” Epidemiol. Infect., vol. 137, no. 3, pp. 307–315, 2009, doi: 10.1017/S0950268808001969.

C. Punsawad, N. Phasuk, K. Thongtup, S. Nagavirochana, and P. Viriyavejakul, “Prevalence of parasitic contamination of raw vegetables in Nakhon Si Thammarat province, southern Thailand,” BMC Public Health, vol. 19, no. 1, pp. 1–7, 2019, doi: 10.1186/s12889-018-6358-9.

World Health Organization, “WHO estimates of the global burden of foodborne diseases. Foodborne disease burden epidemiolgy reference group 2007-2015,” 2015.

J. Ponniah et al., “Listeria monocytogenes in raw salad vegetables sold at retail level in Malaysia,” Food Control, vol. 21, no. 5, pp. 774–778, 2010, doi: 10.1016/j.foodcont.2009.09.008.

S. F. Purba, “Pemeriksaan Escherichia coli dan Larva Cacing pada Sayuran Lalapan Kemangi (Ocimum basilicum), Kol (Brassica oleracea L. var. capitata. L.), Selada (Lactuca sativa L.), Terong (Solanum melongena) yang Dijual di Pasar Tradisional, Supermarket dan Restoran,” Lingkung. dan Kesehat. Kerja, vol. 2, no. 1, p. 14623, 2013.

Dinkes Jawa Timur, “Profil Kesehatan Provinsi Jawa Timur 2019,” Dinas Kesehat. Provinsi Jawa Teng., pp. 1–123, 2020, [Online]. Available: www.dinkesjatengprov.go.id.

N. da Silva, M. H. Taniwaki, V. C. A. Junqueira, N. F. A. Silveira, M. M. Okazaki, and R. A. R. Gomes, Microbiological Examination Methods of Food and Water: A Laboratory Manual, 2nd ed. CRC Press, 2018.

R. Blodgett, “Bacterial Analytical Manual, Appendix 2 Most Probable Number from Serial Dilutions.,” US Food & Drug Administration - FDA. 2010, [Online]. Available: http://www.fda.gov/Fod/FoodScienceResearch/LaboratoryMethods/ucm109656.htm.

J. F. T. K. Akoachere, B. F. Tatsinkou, and J. M. Nkengfack, “Bacterial and parasitic contaminants of salad vegetables sold in markets in Fako Division, Cameroon and evaluation of hygiene and handling practices of vendors,” BMC Res. Notes, vol. 11, no. 1, pp. 1–7, 2018, doi: 10.1186/s13104-018-3175-2.

B. Machado-Moreira, K. Richards, F. Brennan, F. Abram, and C. M. Burgess, “Microbial Contamination of Fresh Produce: What, Where, and How?,” Compr. Rev. Food Sci. Food Saf., vol. 18, no. 6, pp. 1727–1750, 2019, doi: 10.1111/1541-4337.12487.

S. K. Mritunjay and V. Kumar, “A study on prevalence of microbial contamination on the surface of raw salad vegetables,” 3 Biotech, vol. 7, no. 1, pp. 1–9, 2017, doi: 10.1007/s13205-016-0585-5.

G. Alemu, M. Mama, and M. Siraj, “Bacterial contamination of vegetables sold in Arba Minch Town, Southern Ethiopia,” BMC Res. Notes, vol. 11, no. 1, p. 15, 2018, doi: 10.1186/s13104-018-3889-1.

J. Kadariya, T. C. Smith, and D. Thapaliya, “Staphylococcus aureus and Staphylococcal Food-Borne Disease: An Ongoing Challenge in Public Health,” BioMed Research International. 2014, doi: 10.1155/2014/827965.

Y. M. G. Hounmanou et al., “Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: An environmental health study,” BMC Res. Notes, vol. 9, p. 466, 2016, doi: 10.1186/s13104-016-2283-0.

M. S. Najwa et al., “Quantification and antibiotic susceptibility of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium in raw vegetables (ulam),” Int. Food Res. J., vol. 22, no. 5, p. 1761, 2015.

S. Chatterjee and I. H. Raval, “Pathogenic Microbial Genetic Diversity with Reference to Health,” in Microbial Diversity in the Genomic Era, 2018, pp. 559–557.

Y. Wang and X. Pan, “Bacteria: Proteus,” Encycl. Food Saf., vol. 1, pp. 486–489, 2014, doi: 10.1016/B978-0-12-378612-8.00108-6.

BPOM, Pedoman Penerapan Peraturan Badan Pom Tentang Cemaran Mikroba Dalam Pangan Olahan. Jakarta: Direktorat Standardisasi Pangan Olahan Deputi Bidang Pengawasan Pangan Olahan Badan POM R, 2019.

T. Tefera, A. Biruksew, Z. Mekonnen, and T. Eshetu, “Parasitic Contamination of Fruits and Vegetables Collected from Selected Local Markets of Jimma Town, Southwest Ethiopia,” Int. Sch. Res. Not., pp. 1–7, 2014, doi: 10.1155/2014/382715.

Y. Armiyanti, W. S. Utami, Y. Nurdian, J. A. S. Ng, and B. Hermansyah, “Hookworm infection and the risk factors among plantation workers in Jember, Indonesia,” Ann. Trop. Med. Public Heal., vol. 23, no. 8, pp.

–1329, 2020, doi: 10.36295/ASRO.2020.23820.

R. J. Traub, I. D. Robertson, P. Irwin, N. Mencke, and R. C. A. Thompson, “The prevalence, intensities and risk factors associated with geohelminth infection in tea-growing communities of Assam, India,” Trop. Med. Int. Heal., vol. 9, no. 6, pp. 688–701, 2004, doi: 10.1111/j.1365-3156.2004.01252.x.

S. U. Haq, A. Maqbool, U. J. Khan, G. Yasmin, and R. Sultana, “Parasitic contamination of vegetables eaten raw in Lahore,” Pak. J. Zool., vol. 46, no. 5, pp. 1303–1309, 2014.

G. Alemu, M. Nega, and M. Alemu, “Parasitic Contamination of Fruits and Vegetables Collected from Local Markets of Bahir Dar City, Northwest Ethiopia,” Res. Rep. Trop. Med., vol. 11, pp. 17–25, 2020, doi: 10.2147/rrtm.s244737.

G. Kosmadakis, C. Georgoulias, V. Filiopoulos, I. Stefanou, D. Smirloglou, and S. Michail, “Lethal Strongyloides stercoralis superinfection in an immunocompromised patient,” Ren. Fail., vol. 32, no. 6, pp. 750–752, 2010, doi: 10.3109/0886022X.2010.486490.

M. A. Mohamed, E. E. Siddig, A. H. Elaagip, A. M. M. Edris, and A. A. Nasr, “Parasitic contamination of fresh vegetables sold at central markets in Khartoum state, Sudan,” Ann. Clin. Microbiol. Antimicrob., vol. 15, no. 17, pp. 1–7, 2016, doi: 10.1186/s12941-016-0133-5.

A. Bhargava, J. A. Cotton, B. R. Dixon, L. Gedamu, R. M. Yates, and A. G. Buret, “Giardia duodenalis surface cysteine proteases induce cleavage of the intestinal epithelial cytoskeletal protein villin via myosin light chain kinase,” PLoS One, 2015, doi: 10.1371/journal.pone.0136102.

J. Li, Z. Wang, M. R. Karim, and L. Zhang, “Detection of human intestinal protozoan parasites in vegetables and fruits: a review,” Parasites and Vectors, vol. 13, p. 380, 2020, doi: 10.1186/s13071-020-04255-3.

N. Pumipuntu and S. Piratae, “Cryptosporidiosis: A zoonotic disease concern,” Vet. World, vol. 11, no. 5, pp. 681–686, 2018, doi: 10.14202/vetworld.2018.681-686.




DOI: http://doi.org/10.11591/ijphs.v11i4.21875

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