Potential hepatic-protective effect of Physalis Peruviana against Lead-induced Toxicity in Albino Rats

Fatma Ismail, eglal Ghoneim, ossama Abdullatef, samar Aborhyem

Abstract


 

ABSTRACT

 

Lead toxicity poses a serious threat facing humanity. This research aims to investigate the potential hepatoprotective effect of Physalis Peruviana (P.P.) fruit against lead toxicity (10ppm (1/45 LD50)) in male albino rats. Toxicological induction lasted for 28 days followed by treatment with 200, 300, and 500 mg/kg of lyophilized P.P. fruit extract for another 28 days. Antioxidant enzymes including; SOD, MDA, CAT, and GSH using a spectrophotometer and lead concentration in liver tissues were assessed using Atomic Absorption Spectroscopy after 4 and 8 weeks, in addition to liver function ALT and AST, and CBC.  Lead acetate induced substantial elevation in MDA levels, and a significant decrease in SOD, CAT, and GSH activities. Treatment with 200, 300, and 500 mg/kg P.P extracts showed an improvement in the activity of the antioxidant enzymes in a dose-dependent manner. Co-administration of P.P fruit extract ameliorates the damage caused by lead acetate. Physalis Peruviana solution showed a reduction in the mean lead concentration in a dose-dependent response to 0.26±0.02, 0.21±0.02, and 0.14±0.03 ppm for groups III, IV, and V, respectively. In conclusion, P.P fruit extract can induce antioxidant activity and exert a beneficial effect for the treatment of Pb-induced hepatotoxicity.


References


A. T. Jan, M. Azam, K. Siddiqui, A. Ali, I. Choi, and Q. M. Haq, "Heavy Metals and Human Health: Mechanistic Insight into Toxicity and Counter Defense System of Antioxidants," Int. J. Mol. Sci., vol. 16, no. 12, pp. 29592-29630, 2015, doi:10.3390/ijms161226183.

A. E. Abdel Moneim, "Indigofera oblongifolia Prevents Lead Acetate-Induced Hepatotoxicity, Oxidative Stress, Fibrosis and Apoptosis in Rats," PLoS One, vol. 11, no. 7, p. e0158965, 2016, doi:10.1371/journal.pone.0158965.

M. Jaishankar, T. Tseten, N. Anbalagan, B. B. Mathew, and K. N. Beeregowda, "Toxicity, mechanism and health effects of some heavy metals," Interdiscip. Toxicol., vol. 7, no. 2, pp. 60-72, 2014, doi:10.2478/intox-2014-0009.

R. Brochin, S. Leone, D. Phillips, N. Shepard, D. Zisa, and A. Angerio, "The cellular effect of lead poisioning and its clinical picture," Management, vol. 8, no. 1, pp. 1-8, 2014,

E. M. Al-Olayan, M. F. El-Khadragy, A. M. Aref, M. S. Othman, R. B. Kassab, and A. E. Abdel Moneim, "The potential protective effect of Physalis peruviana L. against carbon tetrachloride-induced hepatotoxicity in rats is mediated by suppression of oxidative stress and downregulation of MMP-9 expression," Oxid. Med. Cell Longev., vol. 2014, no., p. 381413, 2014, doi:10.1155/2014/381413.

P. Reckziegel, et al., "Antioxidant protection of gallic acid against toxicity induced by Pb in blood, liver and kidney of rats," Toxicol. Rep., vol. 3, no., pp. 351-356, 2016, doi:10.1016/j.toxrep.2016.02.005.

M. A. Dkhil, S. Al-Quraishy, M. M. Diab, M. S. Othman, A. M. Aref, and A. E. Abdel Moneim, "The potential protective role of Physalis peruviana L. fruit in cadmium-induced hepatotoxicity and nephrotoxicity," Food Chem. Toxicol., vol. 74, no., pp. 98-106, 2014, doi:10.1016/j.fct.2014.09.013.

K. E. Beale, et al., "Accurate measurement of body weight and food intake in environmentally enriched male Wistar rats," Obesity (Silver Spring), vol. 19, no. 8, pp. 1715-1721, 2011, doi:10.1038/oby.2010.331.

A. S. Kanagasabapathy and S. Kumari, "Guidelines on standard operating procedures for clinical chemistry. New Delhi, India: WHO, 2000.

A. Agarwal and A. Majzoub, "Laboratory tests for oxidative stress," Indian J. Urol., vol. 33, no. 3, pp. 199-206, 2017, doi:10.4103/iju.IJU_9_17.

İ. Gulcin, "Antioxidants and antioxidant methods: an updated overview," Arch. Toxicol., vol. 94, no. 3, pp. 651-715, 2020, doi:10.1007/s00204-020-02689-3.

V. Saratha, S. Ambiga, and R. Sangavi, "Evaluation of in vitro antioxidant activity and HPTLC finger printing analysis of Physalis peruviana fruits," Int. J. Pharm. Sci. Drug Res., vol. 12, no. 4, pp. 384-389, 2020, doi:10.25004/IJPSDR.2020.120411.

T. Demİr, M. Ö. Özen, and E. E. Hameş-Kocabaş, "Antioxidant and cytotoxic activity of Physalis peruviana," Med. Plant Res., vol. 4, no. 3, pp. 30-34, 2014,

J. C. Chang, et al., "Antioxidative and Hepatoprotective Effects of Physalis peruviana Extract against Acetaminophen-Induced Liver Injury in Rats," Pharm. Biol., vol. 46, no. 10-11, pp. 724-731, 2008, doi:10.1080/13880200802215768.

N. M. Ibrahim, E. A. Eweis, H. S. El-Beltagi, and Y. E. Abdel-Mobdy, "Effect of lead acetate toxicity on experimental male albino rat," Asian Pac. J. Trop. Biomed., vol. 2, no. 1, pp. 41-46, 2012, doi:10.1016/s2221-1691(11)60187-1.

S. M. Hassan, E. M. El-Kholie, and A. M. Khedr, "Anti-obesity Effect of Gooseberry (Physalis peruviana) Fruits in-Induced Obese Rats," Am. J. Food Nutr., vol. 4, no. 2, pp. 155-170, 2018, doi:10.12691/ajfn-8-4-1.

S. E. El-Gengaihi, E. E. Hassan, M. A. Hamed, H. G. Zahran, and M. A. Mohammed, "Chemical composition and biological evaluation of Physalis peruviana root as hepato-renal protective agent," J. Diet. Suppl., vol. 10, no. 1, pp. 39-53, 2013, doi:10.3109/19390211.2012.760509.

R. Khalaf-Allah Ael, S. E. El-Gengaihi, M. A. Hamed, H. G. Zahran, and M. A. Mohammed, "Chemical Composition of Golden Berry Leaves Against Hepato-renal Fibrosis," J. Diet. Suppl., vol. 13, no. 4, pp. 378-392, 2016, doi:10.3109/19390211.2015.1099584.

M. S. Othman, A. Nada, H. S. Zaki, and A. E. Abdel Moneim, "Effect of Physalis peruviana L. on cadmium-induced testicular toxicity in rats," Biol. Trace Elem. Res., vol. 159, no. 1-3, pp. 278-287, 2014, doi:10.1007/s12011-014-9955-1.

A. S. Ettinger, M. L. Leonard, and J. Mason, "CDC's Lead Poisoning Prevention Program: A Long-standing Responsibility and Commitment to Protect Children From Lead Exposure," J. Public Health Manag. Pract., vol. 25, no., pp. S5-s12, 2019, doi:10.1097/phh.0000000000000868.

S. Bhattacharya, "The role of medicinal plants and natural products in melioration of cadmium toxicity," Orient. Pharm. Exp. Med., vol. 18, no. 3, pp. 177-186, 2018, doi:10.1007/s13596-018-0323-0.

O. M. Ighodaro and O. A. Akinloye, "First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid," Alex. J. Med., vol. 54, no. 4, pp. 287-293, 2018, doi:https://doi.org/10.1016/j.ajme.2017.09.001.

S. Flora and S. Agrawal, "Arsenic, Cadmium, and Lead," in Reproductive and Developmental Toxicology, R. C. Gupta, Ed., 2nd ed United States: Academic Press, 2017, 537-566.

Z. Ding, et al., "Growth, antioxidant capacity, intestinal morphology, and metabolomic responses of juvenile Oriental river prawn (Macrobrachium nipponense) to chronic lead exposure," Chemosphere, vol. 217, no., pp. 289-297, 2019, doi:10.1016/j.chemosphere.2018.11.034.

A. Abdel Moneim and K. M. El-Deib, "The possible protective effects of Physalis peruviana on carbon tetrachloride-induced nephrotoxicity in male albino rats," Life Sci. J., vol. 9, no. 3, pp. 1038-1052, 2012,

A. Y. Al-Brakati, M. S. Fouda, A. M. Tharwat, E. K. Elmahallawy, R. B. Kassab, and A. E. Abdel Moneim, "The protective efficacy of soursop fruit extract against hepatic injury associated with acetaminophen exposure is mediated through antioxidant, anti-inflammatory, and anti-apoptotic activities," Environ. Sci. Pollut. Res. Int., vol. 26, no. 13, pp. 13539-13550, 2019, doi:10.1007/s11356-019-04935-3.

J. R. Stone and S. Yang, "Hydrogen peroxide: a signaling messenger," Antioxid. Redox. Signal., vol. 8, no. 3-4, pp. 243-270, 2006, doi:10.1089/ars.2006.8.243.

G. Flora, D. Gupta, and A. Tiwari, "Toxicity of lead: A review with recent updates," Interdiscip. Toxicol., vol. 5, no. 2, pp. 47-58, 2012, doi:10.2478/v10102-012-0009-2.

N. H. Hsieh, et al., "Anemia risk in relation to lead exposure in lead-related manufacturing," BMC Public Health, vol. 17, no. 1, p. 389, 2017, doi:10.1186/s12889-017-4315-7.

G. Isvoranu, et al., "Toxic effects of non-acute lead exposure on animal model. Bucharest, Romania: National Research and Development Institute for Industrial Ecology, 2019.




DOI: http://doi.org/10.11591/ijphs.v11i4.21737

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